Journal of Advanced Pharmaceutical Science And Technology

Aditya Kumar Singh, Research Scientist-II,
UTMB, Galveston (TX),
USA
Editorial Board

Aditya Kumar Singh ph.D

Research Scientist-II, UTMB, Galveston (TX), USA · United States

Editorial leadership for Journal of Advanced Pharmaceutical Science And Technology ISSN 2328-0182

Research interests

  • Molecular Biology
  • Immunology: Isolation Of Plasmid
  • Genomic Dna
  • Total Rna
  • Rt-Pcr
  • Real-Time Pcr
  • Cloning/Sub Cloning
  • Site-Directed Mutagenesis
  • Vector Design
  • In Vitro Transcription (Using Kits)
  • Transfection Of Cell Line (E.g. Hek) Using Plasmid Dna
  • Microrna Inhibition
  • Microinjection Of Rrna In Xenopus Oocytes
  • Agrobacterium
  • Electroporationand Biolistic-Mediated Transformation
  • Immunohistochemistry
  • Immunoblotting
  • Elisa
  • Ca2+ Overlay
  • Pooldown Assay
  • Northern
  • Southern
  • Western Blotting
  • Protein Expression &Amp; Over-Expression
  • Coip Etc. Cell Biology Techniques: Culturing
  • Maintenance Of The Cell Cultures
  • Cell Lines (Hek) Culture
  • Tissue Culture (Plant
  • Mammalian)
  • Isolation Of Smooth Muscle Cells From Rat Cerebral Resistance Arteries
  • Culture
  • Cell Proliferation Assays
  • Confocal Microscopy
  • Development Of Stable Cell Lines. Microbiological Techniques: Isolation
  • Characterization Of Microbes
  • Maintenance Of Bacterial Culture
  • Bacterial Transformation
  • Microinjection Of Crna In Xenopusoocytes. Radiotracer: Handling Of Radioactive Material
  • Auto Radiography. Biochemical: Protein Purification Either From E. Coli Or Mammalian Cells
  • Affinity Chromatography
  • Ion Exchange Chromatography
  • Hydrophobic Interaction Chromatography (Hic)
  • On-Column Refolding Of Recombinant Proteins
  • Gel Filtration Chromatography
  • Enzyme Kinetics By Using Colorimetric Assay
  • Sds-Page
  • Liposome Preparation
  • Thermal Shift Assay
  • Spr (Biocore)
  • Alphascreen Assay For Protein-Protein Interaction
  • Split-Luciferase Assay (Lca)
  • High Throughput Screening For Small Molecules (Hts)
  • Bioluminescence Assay Etc. Biophysical: Study Of Recombinant Proteins By Using Different Techniques: Isothermal Calorimetry (Itc)
  • Circular Dichroism (Cd)
  • Extrinsic
  • Intrinsic Fluorescence&Nbsp;&Nbsp; Electrophysiological: Study Of Channels Functions In Artificial Lipid Bi-Layer
  • Patch-Clamp Using Xenopusoocytes
  • Hek Cells Myocytes
  • Currently Working On Hek293
  • Brain Slices Patch-Clamp. Computing Experience: Useful Tools Of Microsoft Office Like Word
  • Powerpoint
  • Excel Etc
  • Photoshop
  • Originlab 7.0
  • Corel Draw
  • Grapgpadinstat 3.0 Etc
  • Clamp Fit 9.2 For Electrophysiolocal Analysis
  • Statistical Analysis Programs

Biography

Professional Experience:

  • 2015 - contd. Research Scientist-II, UTMB, Galveston (TX) USA
  • 2013 - 2015 DST Fast Track Young Scientist, NBRI, Lucknow (UP) India
  • 2012 - 2012 Research Associate, NBRI, Lucknow (UP) India
  • 2009 - 2011 Post-doctoral Fellow, Pharmacology, University of Tennessee HSC, Memphis, TN, USA  
  • 2007 - 2009 Post-doctoral Fellow, Cleveland State University, Cleveland, OH, USA  
  • 2006 - 2007 Post-doctoral Fellow (DBT-PDF), CCMB, Hyderabad, AP, India  
  • 2002 - 2006 Senior Research Fellow (CSIR), NBRI, Lucknow, UP, India  
  • 2000 - 2002 Project Research Fellow, NBRI, Lucknow, UP, India  

 Education:

  •  2006 (Ph.D): Doctor of Philosophy in Science with Specialization in Molecular Biology and Genetic Engineering, National Botanical Research Institute (NBRI), and Lucknow University, Lucknow, India  
  • 1998 (MS)  : Master of Science (Life Sciences), 1st Div (70.8%), KN Kaul Institute of Life Sciences, CSJM University, Kanpur, India  
  • 1995 (BS)  : Bachelor of Science, 1st Div (65.92%),  with specialization in Botany, Zoology & Chemistry, SSJM University, Kanpur, India.
  • 1992 (10+2)  : Intermediate, 1st Div (60.0%), Biological Sciences, UP Board, Allahabad.
  • 1990 (10th)  : High School, 1st Div (62.66%), Biological & Mathematical Sciences, UP Board, Allahabad.

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